Anti-NMDAR2B antibody [EPR19295] (ab183942)
![Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258210-anti-nmdar2b-antibody-epr19295-western-blot.jpg "Anti-NMDAR2B antibody [EPR19295] (ab183942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19295] to NMDAR2B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NMDAR2B antibody [EPR19295]
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EPR19295] to NMDAR2B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human hippocampus lysate; Mouse brain and hippocampus lysates; Rat brain and hippocampus lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19295 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse kidney lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5,6 and 7: 30 seconds; Lane 8 and 9: 8 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
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![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-258209-anti-nmdar2b-antibody-epr19295-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat liver lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDa
Exposure time: 8 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
-
![Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.com/ps/products/183/ab183942/Images/ab183942-3-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B antibody [EPR19295] (ab183942)
