Anti-NMDAR2B (phospho S1480) antibody (ab73014)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho S1480)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-NMDAR2B (phospho S1480) antibody
See all NMDAR2B primary antibodies -
Description
Rabbit polyclonal to NMDAR2B (phospho S1480) -
Host species
Rabbit -
Specificity
ab73014 is specific for ~180k NMDAR2B protein phosphorylated at Ser1480. Immunolabeling of the NMDAR2B band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment. -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho-Ser1480 of rat NMDAR2B.
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Positive control
- Rat hippocampal lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73014 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR2B (phospho S1480) antibody (ab73014)All lanes : Anti-NMDAR2B (phospho S1480) antibody (ab73014) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate incubated in lambda-phosphatase (1200 units for 30 min)
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
The phosphospecificity of the labeling is shown in the second lane (lambda-phosphatase). The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
