Anti-Shugoshin antibody (ab225948)
Key features and details
- Rabbit polyclonal to Shugoshin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Shugoshin antibody
See all Shugoshin primary antibodies -
Description
Rabbit polyclonal to Shugoshin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Shugoshin aa 260-439.
Database link: Q5FBB7 -
Positive control
- WB: MCF7 and HeLa whole cell lysates; Mouse brain tissue lysate. ICC/IF: HeLa cells.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Shugoshin antibody (ab225948)All lanes : Anti-Shugoshin antibody (ab225948) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse brain tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 60,64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Shugoshin antibody (ab225948)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Shugoshin (green) using ab225948 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
