Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling MUC13 with ab1231159 at 10.82µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LoVo cell line. The nuclear counterstain is DAPI (blue).
Counterstains:
ab179504  Anti-beta IV Tubulin antibody - Microtubule Marker
ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary.

The negative controls are as follows:

-ve control 1: ab231159 at 1/50 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary at a 1/1000 dilution.

-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution.

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized T84 (human colon epithelial cell) cells labeling MUC13 with ab231159 at a 1.082µg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

Gated on viable cells.