Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGR2 + AGR3 antibody [EPR20164-222]
    See all AGR2 + AGR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human AGR2 His-tagged recombinant protein (aa21-175); Human AGR3 GST-tagged recombinant protein (aa21-166); MCF7, HT-29 and PC-3 whole cell lysates; Mouse and rat stomach lysates; Human lung and stomach lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20164-222

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line.

    Negative control: HeLa (PMID: 16551856).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/10000 dilution

    Lane 1 : Human AGR2 His-tagged recombinant protein (aa21-175)
    Lane 2 : Human AGR3 GST-tagged recombinant protein (aa21-166)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 seconds.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    According to the literature AGR2 is not expressed in HeLa (PMID: 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : Mouse stomach lysate
    Lane 2 : Rat stomach lysate
    Lane 3 : Human lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/2000 dilution + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,18 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
    Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.