Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Aldolase B+Aldolase C antibody [EPR3137]
See all Aldolase B+Aldolase C primary antibodies
Description

Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Aldolase B aa 100-200. The exact sequence is proprietary.
Database link: P05062
Positive control
- Human fetal liver and kidney cell lysates; Human fetal liver tissue; Human adult liver tissue.
General notes
This product was previously labelled as ALDOB+Aldolase C

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3137
Isotype

IgG
Research areas

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag

Lysates/proteins at 0.015 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

Lane 1 : Human fetal liver cell lysate
Lane 2 : Human fetal kidney cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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