Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Aldolase B+Aldolase C antibody [EPR3137]
See all Aldolase B+Aldolase C primary antibodies -
Description
Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Aldolase B aa 100-200. The exact sequence is proprietary.
Database link: P05062 -
Positive control
- Human fetal liver and kidney cell lysates; Human fetal liver tissue; Human adult liver tissue.
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General notes
This product was previously labelled as ALDOB+Aldolase C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3137 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human fetal liver cell lysate
Lane 2 : Human fetal kidney cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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