Anti-NIPBL antibody [KT55] (ab106768)
![Anti-NIPBL antibody [KT55] (ab106768)](https://www.abcam.com/ps/products/106/ab106768/Images/ab106768-195409-anti-idn3-antibody-kt55-flow-cytometry.jpg "Anti-NIPBL antibody [KT55] (ab106768)")
Key features and details
- Rat monoclonal [KT55] to NIPBL
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-NIPBL antibody [KT55]
See all NIPBL primary antibodies -
Description
Rat monoclonal [KT55] to NIPBL -
Host species
Rat -
Specificity
Recognises Human and Mouse NIPBL isoform B (short form).
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NIPBL (C terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from culture supernatant using a protein G column. -
Clonality
Monoclonal -
Clone number
KT55 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-NIPBL antibody [KT55] (ab106768)Overlay histogram showing HepG2 cells stained with ab106768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
