Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14390] to Scc4 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Scc4 antibody [EPR14390] - BSA and Azide free
See all Scc4 primary antibodies -
Description
Rabbit monoclonal [EPR14390] to Scc4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HeLa cells; IP: HeLa whole cell lysate; Flow Cyt (intra): MCF7 cells. WB: HeLa, HT-1080, MCF7, HEK-293, C6, NIH/3T3 and RAW 264.7 whole cell lysate.
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General notes
ab250646 is the carrier-free version of ab183033.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14390 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Scc4 antibody [EPR14390] (ab183033) at 1/5000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDaThis data was developed using ab183033, the same antibody clone in a different buffer formulation.
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This data was developed using ab183033, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling Scc4 with ab183033 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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This data was developed using ab183033, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human 293 lysate immunoprecipitated using ab183033 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
This data was developed using ab183033, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling Scc4 with ab183033 (red) at 1/90 dilution or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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