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Epigenetics and Nuclear Signaling Chromosome Structure Cohesins

Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)

Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14390] to Scc4 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Scc4 antibody [EPR14390] - BSA and Azide free
    See all Scc4 primary antibodies
  • Description

    Rabbit monoclonal [EPR14390] to Scc4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HeLa cells; IP: HeLa whole cell lysate; Flow Cyt (intra): MCF7 cells. WB: HeLa, HT-1080, MCF7, HEK-293, C6, NIH/3T3 and RAW 264.7 whole cell lysate.
  • General notes

    ab250646 is the carrier-free version of ab183033.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14390
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins

Images

  • Western blot - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    Western blot - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    All lanes : Anti-Scc4 antibody [EPR14390] (ab183033) at 1/5000 dilution

    Lane 1 : HT1080 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab183033, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    Immunocytochemistry - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    This data was developed using ab183033, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling Scc4 with ab183033 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Immunoprecipitation - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    Immunoprecipitation - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    This data was developed using ab183033, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human 293 lysate immunoprecipitated using ab183033 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Flow Cytometry - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    Flow Cytometry - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    This data was developed using ab183033, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling Scc4 with ab183033 (red) at 1/90 dilution or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)
    Anti-Scc4 antibody [EPR14390] - BSA and Azide free (ab250646)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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