Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NMDAR2B antibody [NR2B] (ab28373)

Key features and details

  • Mouse monoclonal [NR2B] to NMDAR2B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NMDAR2B antibody [NR2B]
    See all NMDAR2B primary antibodies

  • Description

    Mouse monoclonal [NR2B] to NMDAR2B

  • Host species

    Mouse

  • Specificity

    Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.

  • Positive control

    • WB: Mouse brain tissue ICC: transfected HEK cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

    Predicted band size: 166 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)

    ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
    Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

    Lanes 3 & 6 : Mouse brain (50ug)
    Lanes 4 & 7 : Rat brain (50ug)

    Predicted band size: 166 kDa

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373) This image is courtesy of an anonymous Abreview
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg

    Secondary
    HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa



    Samples were incubated with primary antibody for 12 hours at 4ºC.

    Blocked with 5% milk for 1 hour at 22ºC. 

    See Abreview

  • Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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