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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors

Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)

Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19694] to NMDAR2A - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free
    See all NMDAR2A primary antibodies
  • Description

    Rabbit monoclonal [EPR19694] to NMDAR2A - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250710 is the carrier-free version of ab183939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250710 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19694
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors

Images

  • Western blot - Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
    Western blot - Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
    All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    This data was developed using ab183939, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lanes 3, 4, 5, 6 and 7: 3 minutes.

    NMDAR2A has been previously documented to be specifically expressed in neuron cells (PMID: 12127670).

  • Western blot - Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
    Western blot - Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
    All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution

    Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
    Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    This data was developed using ab183939, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 seconds; Lane 2: 3 minutes.

  • Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
    Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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