Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free (ab250710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19694] to NMDAR2A - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-NMDAR2A antibody [EPR19694] - BSA and Azide free
See all NMDAR2A primary antibodies -
Description
Rabbit monoclonal [EPR19694] to NMDAR2A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250710 is the carrier-free version of ab183939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250710 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19694 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution
Lane 1 : Mouse hippocampus lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaThis data was developed using ab183939, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lanes 3, 4, 5, 6 and 7: 3 minutes.
NMDAR2A has been previously documented to be specifically expressed in neuron cells (PMID: 12127670).
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All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution
Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaThis data was developed using ab183939, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 seconds; Lane 2: 3 minutes.
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