Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2480Y] to NMDAR1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NMDAR1 antibody [EPR2480Y]
    See all NMDAR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2480Y] to NMDAR1

  • Host species

    Rabbit

  • Specificity

    This antibody was raised against a phosphorylated peptide however the antibody recognises phosphorylated and non-phosphorylated protein.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NMDAR1 (phospho S889). The exact sequence is proprietary.
    (Peptide available as ab168728)

  • Positive control

    • Mouse brain lysate; human brain tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2480Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)
    Western blot - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)
    All lanes : Anti-NMDAR1 antibody [EPR2480Y] (ab68144) at 1/1000 dilution

    Lane 1 : Human fetal brain cell lysate
    Lane 2 : Human forebrain cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 105 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68144 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-NMDAR1 antibody [EPR2480Y] (ab68144)
    Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

© 2021 Astana Biomed Group LLP. All rights reserved.