Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2480Y] to NMDAR1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-NMDAR1 antibody [EPR2480Y]
See all NMDAR1 primary antibodies
Description

Rabbit monoclonal [EPR2480Y] to NMDAR1
Host species

Rabbit
Specificity

This antibody was raised against a phosphorylated peptide however the antibody recognises phosphorylated and non-phosphorylated protein.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human NMDAR1 (phospho S889). The exact sequence is proprietary.
(Peptide available as ab168728)
Positive control
- Mouse brain lysate; human brain tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2480Y
Isotype

IgG
Research areas

Western blot - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

All lanes : Anti-NMDAR1 antibody [EPR2480Y] (ab68144) at 1/1000 dilution

Lane 1 : Human fetal brain cell lysate
Lane 2 : Human forebrain cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 105 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68144 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-NMDAR1 antibody [EPR2480Y] (ab68144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com