Anti-NMDAR1 antibody (ab52177)
Key features and details
- Rabbit polyclonal to NMDAR1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR1 antibody
See all NMDAR1 primary antibodies -
Description
Rabbit polyclonal to NMDAR1 -
Host species
Rabbit -
Specificity
ab52177 detects endogenous levels of total NMDAR1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human NMDAR1 aa 849-913. The exact sequence is proprietary. From around the phosphorylation site of serine 897 (R-S-S-K-D).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR1 antibody (ab52177)All lanes : Anti-NMDAR1 antibody (ab52177) at 1/500 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) whole cell lysate
Lane 2 : Y79 (Human retinoblastoma cell line) whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 105 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody (ab52177)ab52177 at 1/50 dilution staining NMDAR1 in human brain tissue by immunohistochemistry (paraffin embedded tissue) in the presence (right image) or absence (left image) of the immunizing peptide.
