Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 123 964 ₸

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Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2481(2)] to NMDAR1
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-NMDAR1 antibody [EPR2481(2)]
See all NMDAR1 primary antibodies
Description

Rabbit monoclonal [EPR2481(2)] to NMDAR1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Mouse

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Fetal brain cell lysate. ICC/IF: Mouse primary neuron cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Mouse
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2481(2)
Isotype

IgG
Research areas

Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

All lanes : Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 105 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling NMDAR1 with purified ab109182 at 1/50 (9.5µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (unpurified) + Human fetal brain cell lysate at 10 µg

Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Secondary antibody - anti-rabbit HRP (ab6721)
Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

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