Anti-NMDAR2B antibody [EPR19295] - BSA and Azide free (ab250711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19295] to NMDAR2B - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NMDAR2B antibody [EPR19295] - BSA and Azide free
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EPR19295] to NMDAR2B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250711 is the carrier-free version of ab183942. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250711 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19295 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse kidney lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDaThis data was developed using ab183942, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-7: 30 seconds; Lanes 8-9: 8 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 9252405; 15470155).
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All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat liver lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDa
Exposure time: 8 secondsThis data was developed using ab183942, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 9252405; 15470155).
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