Anti-NMDAR1 antibody [N308/48] (ab193310)
![Anti-NMDAR1 antibody [N308/48] (ab193310)](https://www.abcam.com/ps/products/193/ab193310/Images/ab193310-287136-anti-nmdar1-antibody-n308-48-immunohistochemistry.jpg "Anti-NMDAR1 antibody [N308/48] (ab193310)")
Key features and details
- Mouse monoclonal [N308/48] to NMDAR1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-NMDAR1 antibody [N308/48]
See all NMDAR1 primary antibodies -
Description
Mouse monoclonal [N308/48] to NMDAR1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHCP - Human Normal Cerebral Cortex
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N308/48 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody [N308/48] (ab193310)
IHC image of NMDAR1 staining in Human Normal Cerebral Cortex formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab193310, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-NMDAR1 antibody [N308/48] (ab193310)](https://www.abcam.com/ps/products/193/ab193310/Images/ab193310-244023-anti-nmdar1-antibody-n308-48-western-blot.jpg)
Western blot - Anti-NMDAR1 antibody [N308/48] (ab193310)All lanes : Anti-NMDAR1 antibody [N308/48] (ab193310) at 1 µg/ml
Lanes 1 & 6 : Brain (Mouse) Tissue Lysate
Lanes 2 & 7 : Brain (Rat) Tissue Lysate
Lanes 3 & 8 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lanes 4 & 9 : Spinal Cord (Mouse) Tissue Lysate
Lanes 5 & 10 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 105 kDa
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesLanes 1-5 were blocked with 2% BSA.
Lanes 6-10 were blocked with 3% milk.
We recommend using 3% milk as the blocking agent for Western blot.
