Anti-RAD18 antibody (ab188235)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAD18 antibody
See all RAD18 primary antibodies -
Description
Rabbit polyclonal to RAD18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RAD18 aa 250 to the C-terminus.
Database link: Q9NS91 -
Positive control
- Irradiated U2OS cells and lysate, NIH-3T3 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAD18 antibody (ab188235)All lanes : Anti-RAD18 antibody (ab188235) at 1/2000 dilution
Lane 1 : Control U2OS cell lysate
Lane 2 : RAD18 si treated U2OS cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 56 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab188235)Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab188235)Immunofluorescent analysis of RAD18 expression in U2OS control cells and RAD18 siRNA treated U2OS cells, using ab188235 at 1/2000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab188235)Immunofluorescent analysis U2OS cells fixed in 3% PFA 10 mins and permeabilised with methanol 2 mins irradiated with 3Gy IR for 1.5h labeling RAD18 with ab188235 (1), DNA (2) and merged (3)
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Immunoprecipitation - Anti-RAD18 antibody (ab188235)Western blot analysis (using ab188235) of immunoprecipitation from U2OS cell lysates(lysated in 0.5% NP40)
Lane 1: Input
Lane 2: immunoprecipitate with control serum+protein A sepharose.
Lane 3: immunoprecipitate with ab188235+ protein A sepharose.
