Anti-RAD18 antibody (ab186835)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAD18 antibody
See all RAD18 primary antibodies -
Description
Rabbit polyclonal to RAD18 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human RAD18 aa 1-495.
Database link: Q9NS91 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab186835)
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using ab186835. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab186835)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186835. Blue DAPI for nuclear staining. -
Western blot - Anti-RAD18 antibody (ab186835)All lanes : Anti-RAD18 antibody (ab186835) at 1/500 dilution
Lane 1 : Extracts from K562 cell line
Lane 2 : Extracts from MCF7 cell line
Lane 3 : Extracts from Jurkat cell line
Lane 4 : Extracts from 293T cell line
Lane 5 : Extracts from mouse liver
Predicted band size: 56 kDa
