RAD18 overexpression 293T lysate (whole cell) (ab94312)
Overview
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Product name
RAD18 overexpression 293T lysate (whole cell) -
General notes
ab94312 is a 293T cell transfected lysate in which Human RAD18 has been transiently over-expressed using a pCMV-RAD18 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.
Images
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SDS-PAGE - RAD18 overexpression 293T lysate (whole cell) (ab94312)ab94312 at 15μg/lane on an SDS-PAGE gel
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Western blot - RAD18 overexpression 293T lysate (whole cell) (ab94312)All lanes : Anti-RAD18 antibody (ab57447) at 1/500 dilution
Lane 1 :RAD18 overexpression 293T lysate (whole cell) (ab94312)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
