Anti-RAD18 antibody - BSA and Azide free (ab188283)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-RAD18 antibody - BSA and Azide free
See all RAD18 primary antibodies -
Description
Rabbit polyclonal to RAD18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse RAD18 (C terminal). 100 carboxyl terminal amino acids of mouse Rad18 fused to a propriatary tag
Database link: Q9QXK2 -
Positive control
- ES, NIH 3T3 cell lysates; ES cells; mouse testis.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
Filter-sterilized -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAD18 antibody - BSA and Azide free (ab188283)All lanes : Anti-RAD18 antibody - BSA and Azide free (ab188283) at 1/1000 dilution
Lane 1 : Lysate of wild type mouse ES cells
Lane 2 : Lysate of Rad18 double knock-out mouse ES cells
Predicted band size: 56 kDa
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Western blot - Anti-RAD18 antibody - BSA and Azide free (ab188283)Anti-RAD18 antibody - BSA and Azide free (ab188283) at 1/2000 dilution + NIH 3T3 cell extract at 23 µg
Predicted band size: 56 kDaThe two extra bands (75~90 kDa) may represent modified products
(ubiquitination, phosphorylation). -
Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody - BSA and Azide free (ab188283)Immunocytochemical analysis of wild-type (1) and RAD18 -/- (2) cells fixed in 3.7% paraformaldehyde and 80% methanol, labeling RAD18 with ab188283 at a 1/300 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD18 antibody - BSA and Azide free (ab188283)Immunohistochemical analysis of paraformaldehyde fixed mouse testis labeling RAD18 with ab188283 at 1/100 dilution. Peroxidase-conjugated anti-rabbit IgG donkey antibody was used as the second antibody. Signals were enhanced with TSA plus biotin system and detected using DAB substrate. Arrows indicate undifferentiated spermatogonia.
