Anti-NMDAR2A (phospho Y1325) antibody (ab106590)
Key features and details
- Rabbit polyclonal to NMDAR2A (phospho Y1325)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies -
Description
Rabbit polyclonal to NMDAR2A (phospho Y1325) -
Host species
Rabbit -
Specificity
Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide. -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.
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Positive control
- Rat hippocampal lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab106590)All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide
Predicted band size: 165 kDa
Observed band size: ~180 kDa why is the actual band size different from the predicted?
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Western blot - Anti-NMDAR2A (phospho Y1325) antibody (ab106590) This image is courtesy of an Abreview submitted by Dr Xiaofan WangAll lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution
All lanes : Rat brain whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
