Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18945] to USP22 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP22 antibody [EPR18945] - BSA and Azide free
    See all USP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR18945] to USP22 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, fetal heart and fetal kidney lysates; HeLa, HEK-293, Jurkat, HepG2, MCF7, Neuro-2a, F9, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain and spleen lysates; Rat brain and spleen lysates. IP: HeLa whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissue; Human breast carcinoma tissue.

  • General notes

    Ab238948 is the carrier-free version of ab195289. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Western blot - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: USP22 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HEK293 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab195289 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab195289 was shown to specifically react with USP22 in wild-type HAP1 cells. No band was observed when knockout samples were examined. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. Ab195289 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at a 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling USP22 with ab195289 at 1/1000 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Nuclear staining on rat cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling USP22 with ab195289 at 1/1000 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Nuclear staining on mouse cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling USP22 with ab195289 at 1/100 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Nuclear staining on human breast carcinoma. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Immunohistochemistry paraffin embedded sections - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling USP22 with ab195289 at 1/100 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use dilution. Nuclear staining on human cerebrum. The section was incubated with ab195289 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Immunoprecipitation - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Immunoprecipitation - Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

    USP22 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab195289 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab195289 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10µg (Input).

    Lane 2: ab195289 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab195289 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195289).

  • Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)
    Anti-USP22 antibody [EPR18945] - BSA and Azide free (ab238948)

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