Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)
![Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)](https://www.abcam.com/ps/products/206/ab206035/Images/ab206035-295742-anti-lrrk2-phospho-s1292-antibody-mjfr-19-7-8-western-blot.jpg "Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292) - Low endotoxin, Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free
See all LRRK2 primary antibodies -
Description
Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292) - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; GFP-LRRK2 mutant G 2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; LRRK2 wt transfected HEK293 lysate; LRRK2 mutant G2019S transfected HEK293 lysate.
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General notes
ab206035 is the Low endotoxin, azide-free version of ab203181 This format is designed for in vitro and in vivo studies, including neutralization, blocking or activation/proliferation.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJFR-19-7-8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml
Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution
Predicted band size: 286 kDaThis WB data was generated using the sam anti-LRRK2 (phospho S1292) antibody clone, MJFR-19-7-8, in a different buffer formulation (cat# ab203181).
Blocking/Dilution buffer: 5% BSA/TBST.
The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.
G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).
Observed band size: 286 + 27 (GFP) = 313 kDa.
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![Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)](https://www.abcam.com/ps/products/206/ab206035/Images/ab206035-3-benefits-of-recombinant-antibodies.png)
Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)
