Recombinant Human Alpha-synuclein protein monomer (Active) (ab218818)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
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Product name
Recombinant Human Alpha-synuclein protein monomer (Active)
See all Alpha-synuclein proteins and peptides -
Biological activity
100 µM alpha synuclein protein monomer (ab218818), seeded with 10 µM alpha synuclein protein aggregate in 25 µM Thioflavin T (ab120751) (PBS pH 7.4, 100 µl reaction volume), generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.
Fluorescence was measured by excitation at 450 nm and emission at 485 nm, on a Molecular Devices Gemini XPS microplate reader.
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Purity
> 95 % SDS-PAGE.
ab218818 is purified by ion exchange. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA -
Predicted molecular weight
14 kDa -
Amino acids
1 to 140 -
Additional sequence information
(NP_000336.1) (GeneID 6622)
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Description
Recombinant human Alpha-synuclein protein (Active)
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant Human Alpha-synuclein protein monomer (Active) (ab218818)SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer (SPR-321). Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 µg) (SPR-321).
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Functional Studies - Recombinant Human Alpha-synuclein protein monomer (Active) (ab218818)ThT emission curves show increased fluorescence (correlated to alpha-synuclein protein aggregation) over time when 10 µM of active alpha-synuclein aggregate (ab218819) is combined with 100 µM of active alpha-synuclein monomer (ab218818) (light blue), as compared to when 100 µM of control alpha-synuclein monomer (ab218816) is combined with 10 µM of active alpha-synuclein aggregate (ab218819) (dark blue).
ThT ex = 450 nm, em = 485 nm. View protocol.
