Recombinant Human Alpha-synuclein protein aggregate (Type 2) (ab218817)
Key features and details
- Expression system: Escherichia coli
- Purity: = 92% SDS-PAGE
- Endotoxin level:
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.24% Tris, 0.87% Sodium chloride
0.2µm filtered solution
Images
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Functional Studies - Recombinant Human Alpha-synuclein protein aggregate (Type 2) (ab218817)
ThT emission curves show increased fluorescence (correlated to alpha-synucleinprotein aggregation) over time when 10 µM of active alpha-synuclein aggregate (ab218819) is combined with 100 µM of active alpha-synuclein monomer (ab218818) (light blue), as compared to when 100 µM of active alpha-synuclein monomer is combined with 10 µM of control alpha-synuclein aggregate (purple line), or 100 µM of control alpha-synuclein monomer (ab218816) is combined with 10 µM of control alpha-synuclein aggregate (ab218817) (dark blue). ThT ex = 450 nm, em = 485 nm. View protocol.
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SDS-PAGE - Recombinant Human Alpha-synuclein protein aggregate (Type 2) (ab218817)SDS-PAGE analysis of ab218817.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Human Alpha-synuclein protein aggregate (Type 2) (ab218817)Immunohistochemical analysis of 4% formaldehyde fixed primary rat hippocampal neurons from Sprague-Dawley rat labeling lewy body inclusions formed when treated with active Alpha Synuclein Protein Aggregate at 4 µg/ml (D-F), but not when treated with ab218817 at 4 µg/ml (A-C).
Primary antibody: Mouse anti-pSer129 antibody at 1/1000 24 hours at 4°C. Secondary antibody: FITC Goat Anti-Mouse (green) at 1/700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1/4000 for 1 hour at RT. Magnification: 20x.
