Synphilin 1 overexpression 293T lysate (whole cell) (ab94102)
Overview
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Product name
Synphilin 1 overexpression 293T lysate (whole cell) -
General notes
ab94102 is a 293T cell transfected lysate in which Human Synphilin 1 has been transiently over-expressed using a pCMV-Synphilin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Disease: Parkinson disease Function: Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1. PTM: Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation. Similarity: Contains 6 ANK repeats. Tissue specificity: Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
Images
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SDS-PAGE - Synphilin 1 overexpression 293T lysate (whole cell) (ab94102)ab94102 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Synphilin 1 overexpression 293T lysate (whole cell) (ab94102)All lanes : Anti-Synphilin 1 antibody (ab56627) at 1/500 dilution
Lane 1 :Synphilin 1 overexpression 293T lysate (whole cell) (ab94102)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution