Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3307(2)] to VCP - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VCP antibody [EPR3307(2)] - BSA and Azide free
    See all VCP primary antibodies

  • Description

    Rabbit monoclonal [EPR3307(2)] to VCP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab184905 is the carrier-free version of ab109240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab184905 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Immunoprecipitation - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    ab109240 (purified) at 1:20 dilution (2ug) immunoprecipitating VCP in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab109240 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109240 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

  • Flow Cytometry - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Flow Cytometry - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Flow Cytometry analysis of HL-60 (Human acute promyelocytic leukemia promyeloblast) cells labeling VCP with purified ab109240 at 1:300 dilution (1 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black).Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling VCP with Purified ab109240 at 1:500 dilution (0.7μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling VCP with purified ab109240 at 1:250 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109240 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Immunofluorescent staining of HeLa cells using unpurified ab109240 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

  • Flow Cytometry - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Flow Cytometry - Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

    Overlay histogram showing HL60 cells stained with  unpurified ab109240 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109240, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109240).

  • Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)
    Anti-VCP antibody [EPR3307(2)] - BSA and Azide free (ab184905)

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