Human gamma-synuclein ELISA Kit (ab224884)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.15 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Human
Overview
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Product name
Human gamma-synuclein ELISA Kit
See all gamma Synuclein/SNCG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.1% Inter-assay Sample n Mean SD CV% Serum 3 14% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Semi-quantitative -
Sensitivity
5.15 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 107 100% - 114% Serum 100 90% - 105% Hep Plasma 89 83% - 92% EDTA Plasma 73 66% - 77% Cit plasma 79 75% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human gamma-synuclein ELISA Kit (ab224884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of gamma-synuclein protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human gamma-synuclein with 5.15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human gamma-synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml 10X Human gamma-synuclein Capture Antibody 1 x 600µl 10X Human gamma-synuclein Detector Antibody 1 x 600µl Human gamma-synuclein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. -
Tissue specificity
Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. -
Sequence similarities
Belongs to the synuclein family. -
Post-translational
modificationsPhosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. - Information by UniProt
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Alternative names
- BCSG1
- Breast cancer specific gene 1 protein
- Breast cancer specific protein 1
see all -
Database links
- Entrez Gene: 6623 Human
- Omim: 602998 Human
- SwissProt: O76070 Human
- Unigene: 349470 Human
Images
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Other - Human gamma-synuclein ELISA Kit (ab224884)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human gamma-synuclein standard curve in Sample Diluent NSThe gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human gamma-synuclein standard curve in Sample Diluent PTRThe gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native gamma-synuclein in human serum and plasma samplesThe concentrations of gamma-synuclein were measured in duplicate, interpolated from the gamma-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 126.89 pg/mL in serum, 114.40 pg/mL in plasma (citrate), 78.63 pg/mL in plasma (EDTA) and 173.43 pg/ml in plasma (heparin).
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Interpolated concentrations of native gamma-synuclein in human urine (from a bladder cancer patient) and cell culture extract based on a 5 µg/mL loadThe concentrations of gamma-synuclein were measured in duplicate and interpolated from the gamma-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 2193.46 pg/mL in bladder cancer urine, 316.85 pg/ml in HT-29 cell extract.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 180.4 pg/mL with a range of 32.92 - 451.67 pg/mL.
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Urine from pooled normal human and four individual bladder cancer patients (male: n=3; female: n=1) human donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 569.88 pg/mL with a range of 40.46 – 2021.56 pg/ml. The native concentration of gamma-synuclein was below the level of detection in urine from healthy human donors.
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Sandwich ELISA - Human gamma-synuclein ELISA Kit (ab224884)To learn more about the advantages of recombinant antibodies see here.