Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)
![Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)](https://www.abcam.com/ps/products/186/ab186334/Images/ab186334-312789-anti-lrrk2-antibody-n138-6-n-terminal-immunofluorescence.jpg "Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)")
Key features and details
- Mouse monoclonal [N138/6] to LRRK2 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-LRRK2 antibody [N138/6] - N-terminal
See all LRRK2 primary antibodies -
Description
Mouse monoclonal [N138/6] to LRRK2 - N-terminal -
Host species
Mouse -
Specificity
No significant identity with LRRK1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human LRRK2 aa 1-500 (N terminal). NP_940980.
Database link: Q5S007 -
Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK- N-BE cells.
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General notes
The clone number has been updated from S138-6 to N138/6, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N138/6 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)
4% formaldehyde-fixed SK-N-BE cells stained for LRRK2 (green) using ab186334 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 minutes room temperature, 5 minutes room temperature. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab186334 (D) Composite.
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![Western blot - Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)](https://www.abcam.com/ps/products/186/ab186334/Images/ab186334-312788-anti-lrrk2-antibody-n138-6-n-terminal-western-blot.jpg)
Western blot - Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334)Anti-LRRK2 antibody [N138/6] - N-terminal (ab186334) at 1/200 dilution + Rat brain membrane lysate
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Predicted band size: 286 kDa
