Anti-VCP antibody [3E8DC11] (ab110308)
![Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123580-anti-vcp-antibody-3e8dc11-immunofluorescence.jpg "Anti-VCP antibody [3E8DC11] (ab110308)")
Key features and details
- Mouse monoclonal [3E8DC11] to VCP
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-VCP antibody [3E8DC11]
See all VCP primary antibodies -
Description
Mouse monoclonal [3E8DC11] to VCP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP MouseRatHuman
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: Human HDFn cells IP: Human, Rat, and Mouse liver samples; HepG2 cultured cell lysate Flow Cyt: HL-60 cells
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The purity of ab110308 is near homogeneity, as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3E8DC11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VCP antibody [3E8DC11] (ab110308)Immunocytochemistry image of ab110308-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110308 at 2 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in nucleus and cytoplasm.
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![Immunoprecipitation - Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123579-anti-vcp-antibody-3e8dc11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VCP antibody [3E8DC11] (ab110308)ab110308 pulls down the 89 kDa VCP protein in Human (lane 1), Rat (lane 2), and Mouse (lane 3) liver samples and Human HepG2 cultured cell lysate (lane 4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with sypro ruby gel stain. -
![Flow Cytometry - Anti-VCP antibody [3E8DC11] (ab110308)](https://www.abcam.com/ps/products/110/ab110308/Images/ab110308-123578-anti-vcp-antibody-3e8dc11-flow-cytometry.jpg)
Flow Cytometry - Anti-VCP antibody [3E8DC11] (ab110308)HL-60 cells were stained with 1 µg/mL ab110308 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
