HRP Anti-VCP antibody [EPR3307(2)] (ab204290)
![HRP Anti-VCP antibody [EPR3307(2)] (ab204290)](https://www.abcam.com/ps/products/204/ab204290/Images/ab204290-265779-anti-vcp-antibody-epr33072-hrp-immunohistochemistry.jpg "HRP Anti-VCP antibody [EPR3307(2)] (ab204290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3307(2)] to VCP
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-VCP antibody [EPR3307(2)]
See all VCP primary antibodies -
Description
HRP Rabbit monoclonal [EPR3307(2)] to VCP -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide within Human VCP. The exact sequence is proprietary.
Database link: P55072 -
Positive control
- WB: MCF-7, HeLa, A549 and SH-SY5Y whole cell lysates. IHC-P: FFPE rat brain (normal) tissue sections.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3307(2) -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCP antibody [EPR3307(2)] (ab204290)
IHC image of VCP staining in a section of formalin-fixed paraffin-embedded normal rat brain tissue, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab204290, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
![Western blot - HRP Anti-VCP antibody [EPR3307(2)] (ab204290)](https://www.abcam.com/ps/products/204/ab204290/Images/ab204290-257505-anti-vcp-antibody-epr33072-hrp-western-blot.jpg)
Western blot - HRP Anti-VCP antibody [EPR3307(2)] (ab204290)All lanes : HRP Anti-VCP antibody [EPR3307(2)] (ab204290) at 1/5000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204290 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-VCP antibody [EPR3307(2)] (ab204290)](https://www.abcam.com/ps/products/204/ab204290/Images/ab204290-4-benefits-of-recombinant-antibodies.png)
HRP Anti-VCP antibody [EPR3307(2)] (ab204290)
