Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

500 µl 278 083 ₸

Order now and get it on Friday March 05, 2021

Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Key features and details

Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human
Isotype: IgG2a

Product name

Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal
Description

Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
Host species

Mouse
Specificity

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
Database link: Q92731
Positive control
- Flow Cyt: BT474 and MCF-7 cells IHC-P: Ovarian carcinoma, breast carcinoma tissues, gastric carcinoma, and bladder carcinoma tissues. WB: MCF7 cell lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

ab187291 was purified from Bioreactor concentrate.
Clonality

Monoclonal
Clone number

ERb455
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.

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