Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
![Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-220890-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg "Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)")
Key features and details
- Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal -
Description
Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal -
Host species
Mouse -
Specificity
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP Human
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Immunogen
Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
Database link: Q92731 -
Positive control
- Flow Cyt: BT474 and MCF-7 cells IHC-P: Ovarian carcinoma, breast carcinoma tissues, gastric carcinoma, and bladder carcinoma tissues. WB: MCF7 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab187291 was purified from Bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
ERb455 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-454709-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-western-blot-mcf7-human.jpg)
Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 59 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-220889-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-252565-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-252568-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-251289-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.
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![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-251287-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.