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Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [CRET94D] to Estrogen Receptor alpha - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free
    See all Estrogen Receptor alpha primary antibodies

  • Description

    Mouse monoclonal [CRET94D] to Estrogen Receptor alpha - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human breast cancer tissue, mouse breast tissue and mouse ovary tissue lysates. IHC: Human breast carcinoma and mouse breast tissue.

  • General notes

    ab252794 is the PBS only format of ab241557.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product has switched from a hybridoma to recombinant production method on 07th December 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).

    Immunohistochemical analysis of paraffin-embedded mouse breast labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma and mouse breast tissues labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
    Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
    All lanes : Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557) at 1/1000 dilution

    Lane 1 : Human breast cancer tissue lysates
    Lane 2 : Mouse breast tissue lysates
    Lane 3 : Mouse ovary tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368)

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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