Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Proteasome 20S LMP2 antibody [EPR13785]
    See all Proteasome 20S LMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, A431 and Raji cell lysates; HL60 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13785

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
    Lanes 1 - 2: Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    All lanes : Anti-EXTL1 antibody - C-terminal (ab174172) at 1/100000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

    Immunoprecipitation analysis of Raji cells labeling Proteasome 20S LMP2 using ab184172 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Raji lysates. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)
    Anti-Proteasome 20S LMP2 antibody [EPR13785] (ab184172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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