Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AATF/DED antibody [EPR22934-136] (ab233546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22934-136] to AATF/DED
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-AATF/DED antibody [EPR22934-136]
    See all AATF/DED primary antibodies

  • Description

    Rabbit monoclonal [EPR22934-136] to AATF/DED

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7, NIH/3T3, HEK-293T, HeLa and HepG2 whole cell lysates. ICC/IF: HEK-293T and NIH/3T3 cells. IP: HEK-293T whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (ab233546)

    Immunofluorescent analysis of 4% paraformaldehyde -fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling AATF/DED with ab233546 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in HEK-293T cells. The nuclear counte stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by  Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Immunoprecipitation - Anti-AATF/DED antibody [EPR22934-136] (ab233546)

    AATF/DED was immunoprecipitated from 0.35 mgof HEK-293T  (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab233546 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233546 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: HEK-293T whole cell lysate 10 μg (Input).
    Lane 2: ab233546 IP in HEK-293T whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233546 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

  • Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    All lanes : Anti-AATF/DED antibody [EPR22934-136] (ab233546) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19911006, 22933572).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Immunocytochemistry/ Immunofluorescence - Anti-AATF/DED antibody [EPR22934-136] (ab233546)

    Immunofluorescent analysis of 4% paraformaldehyde -fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling AATF/DED with ab233546 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining in NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by  Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Western blot - Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    All lanes : Anti-AATF/DED antibody [EPR22934-136] (ab233546) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19911006, 22933572).

     

  • Anti-AATF/DED antibody [EPR22934-136] (ab233546)
    Anti-AATF/DED antibody [EPR22934-136] (ab233546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AATF/DED antibody [EPR22934-136] (ab233546)

© 2021 Astana Biomed Group LLP. All rights reserved.