Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21188] to Myelin Basic Protein - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free
    See all Myelin Basic Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR21188] to Myelin Basic Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum tissue.

  • General notes

    Ab230378 is the carrier-free version of ab218011. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain (cerebrum) tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining in the axonal fiber tracts on rat cerebrum tissue section (PMID: 23144976) is observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris-EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218011).

  • Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (cerebrum) tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining in the axonal fiber tracts on rat cerebrum tissue section (PMID: 23144976) is observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris-EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218011).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on rat brain (PMID: 23144976) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218011).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on mouse brain (PMID: 23144976) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218011).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human cerebrum (PMID: 22496821) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218011).

  • Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)
    Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free (ab230378)

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