Anti-Myelin Basic Protein antibody (ab2404)
Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Myelin Basic Protein antibody
See all Myelin Basic Protein primary antibodies -
Description
Rabbit polyclonal to Myelin Basic Protein -
Host species
Rabbit -
Specificity
This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves. -
Tested applications
Suitable for: IHC-FoFr, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Human myelin basic protein isolated from normal human brain.
-
Positive control
- Brain and spinal cord.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Proprietary Purification -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)ab2404 staining Myelin Basic Protein in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry. Staining was detected using DAB. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404) Image courtesy of Dr Pezet, CARD Institute, KCL, London, UKab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30
µ Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404) Image courtesy of Dr Pezet, CARD institute, KCL, London, UKab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
