Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
![Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147500-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg "Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6652] to Myelin Basic Protein
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Myelin Basic Protein antibody [EPR6652]
See all Myelin Basic Protein primary antibodies -
Description
Rabbit monoclonal [EPR6652] to Myelin Basic Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P02686-1 -
Positive control
- Human fetal brain lysate, Myelin Basic Protein recombinant protein, Human oligodendroglioma and Human brain tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6652 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147497-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing positive staining in Astrocytoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147502-anti-myelin-basic-protein-antibody-epr6652-western-blot.jpg)
Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Human fetal brain lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147499-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147501-anti-myelin-basic-protein-antibody-epr6652-western-blot.jpg)
Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Myelin Basic Protein recombinant protein at 0.01 µg
Predicted band size: 33 kDa
Note: the molecular weight of the recombinant protein is 46 kDa. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147498-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147496-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-8-benefits-of-recombinant-antibodies.png)
Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
