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Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1045Y] to ErbB2 / HER2
  • Suitable for: ICC, IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y]
    See all ErbB2 / HER2 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1045Y] to ErbB2 / HER2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: human breast cancer tissue and SK-BR-3 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Images

  • Immunocytochemistry - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)
    Immunocytochemistry - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-BR-3 cells labelling ErbB2 / HER2 with ab275994 at 1/50 (10.0 ug/ml) dilution, followed by antibody at 1/None dilution (Green). Confocal image showing membranous staining in SK-BR-3 cells ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling ErbB2 / HER2 with ab275994 at 1/100 (5.0 ug/ml) dilution followed by a ready to use . Negative control: no staining on human normal breast. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with 275994 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount.Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

    Secondary antibody only control: Secondary antibody is a ready to use .

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB2 / HER2 with ab275994 at 1/100 (5.0 ug/ml) dilution followed by a ready to use . Specific membranous staining on the human breast carcinoma.The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with 275994 at 1/100 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount.Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

    Secondary antibody only control: Secondary antibody is a ready to use .

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)
    Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)

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