Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Human ALPP (PLAP) knockout HeLa cell lysate (ab256836)

Price and availability

331 689 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Human ALPP (PLAP) knockout HeLa cell lysate (ab256836)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
FITC Anti-CD98 antibody [MEM-108] (ab26010)
Product image
Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)
Product image
Recombinant Human Heparanase 1 protein (denatured) (ab181927)
Product image
Alexa Fluor® 488 Anti-GRP94 antibody [EPR3988] (ab199042)

Overview

  • Product name

    Human ALPP (PLAP) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 5Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 5.
  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260133 - Human ALPP knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Alkaline Phosphatase
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Target

  • Sequence similarities

    Belongs to the alkaline phosphatase family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P05187 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Alkaline phosphatase
    • Alkaline phosphatase placental
    • Alkaline phosphatase placental type
    • Alkaline phosphatase Regan isozyme
    • ALP
    • Alp1
    • ALPP
    • FLJ61142
    • Germ-cell alkaline phosphatase
    • nagao isozyme
    • OTTHUMP00000164354
    • PALP
    • Placental alkaline phosphatase 1
    • placental heat-stable alkaline phosphatase
    • placental type
    • PLAP
    • PLAP-1
    • PLAP1
    • PPB1_HUMAN
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260133 - Human ALPP knockout HeLa cell lysate 1 x 100µg
    ab255552 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Alkaline Phosphatase
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Images

  • Western blot - Human ALPP knockout HeLa cell lysate (ab256836)
    Western blot - Human ALPP knockout HeLa cell lysate (ab256836)

    Lane 1: Wild-type HeLa cell lysate (20 µg)

    Lane 2: ALPP knockout HeLa cell lysate (20 µg)

    Lane 3: Human placenta tissue lysate (20 µg)

    Lane 4: Human heart tissue lysate (20 µg)

    Lanes 1-4: Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used.Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Sanger Sequencing - Human ALPP knockout HeLa cell lysate (ab256836)
    Sanger Sequencing - Human ALPP knockout HeLa cell lysate (ab256836)
    Homozygous: 17 bp deletion in exon 5

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Recombinant Human TGF beta 2 protein (ab159690)

  •  
  • Product image

    Recombinant Human HSD17B14 protein (ab101209)

  •  
  • Product image

    Human GOLGA5 (Golgin-84) knockout HEK-293T cell line (ab267252)

  •  
  • Product image

    Human MUC1 knockout HeLa cell line (ab255412)

  •  
  • Product image

    Recombinant BH0637 protein (Tagged) (ab239565)

  •  
  • Product image

    Recombinant Human Stanniocalcin 1/STC protein (Tagged) (ab238347)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.