(S)-3,5-DHPG, group I mGlu agonist (ab120007)
Key features and details
- Selective group I mGlu agonist
- CAS Number: 162870-29-3
- Purity: > 99%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
(S)-3,5-DHPG, group I mGlu agonist -
Description
Selective group I mGlu agonist -
Biological description
Selective group I mGlu agonist.
Also available in simple stock solutions (ab144484) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 99% -
CAS Number
162870-29-3 -
Chemical structure
Properties
-
Chemical name
(S)-3,5-Dihydroxyphenylglycine -
Molecular weight
183.16 -
Molecular formula
C8H9NO4 -
PubChem identifier
443586 -
Storage instructions
Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes. -
Solubility overview
Soluble in water to 50 mM -
Handling
This compound is also unstable in alkaline solution when it rapidly forms a brown colouration it is therefore recommended that alkaline is not used to dissolve this compound.
We recommend stock solutions are made up in water, aliquoted immediately and stored at -20°C. They should be used within one week.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
Oc1cc(cc(O)c1)[C@H](N)C(=O)O -
Source
Synthetic
-
Research areas
Images
-
Functional Studies - (S)-3,5-DHPG, group I mGlu agonist (ab120007)
SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (S)-3,5-DHPG (ab120007). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase (S)-3,5-DHPG concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.