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Epigenetics and Nuclear Signaling Transcription Other factors

Human CEBPB Antibody Pair - BSA and Azide free (ab244103)

Human CEBPB Antibody Pair - BSA and Azide free (ab244103)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Anti-CEBP Beta antibody [E299] - BSA and Azide free (ab220813)

Overview

  • Product name

    Human CEBPB Antibody Pair - BSA and Azide free
    See all CEBP Beta kits
  • Assay type

    ELISA set
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CEBPB. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab233631), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CEBPB Capture Antibody (unconjugated) 1 x 100µg
    Human CEBPB Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
  • Function

    Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificity

    Expressed at low levels in the lung, kidney and spleen.
  • Sequence similarities

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17676 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AGP/EBP
    • C EBP beta
    • C/EBP beta
    • C/EBP related protein 2
    • CCAAT Enhancer Binding Protein beta
    • CCAAT/enhancer-binding protein beta
    • CEBPB
    • CEBPB_HUMAN
    • CRP2
    • IL 6DBP
    • IL6DBP
    • Interleukin 6 dependent binding protein
    • LAP
    • Liver activator protein
    • Liver enriched transcriptional activator
    • NF IL6
    • NFIL6
    • Nuclear factor NF IL6
    • Nuclear factor NF-IL6
    • SF B
    • SFB
    • Silencer factor B
    • TCF-5
    • TCF5
    • Transcription factor 5
    see all
  • Database links

    • Entrez Gene: 1051 Human
    • Omim: 189965 Human
    • SwissProt: P17676 Human
    • Unigene: 517106 Human
    • Unigene: 719041 Human
    • Unigene: 720603 Human

    Images

    • Sandwich ELISA - Human CEBPB Antibody Pair - BSA and Azide free (ab244103)
      Sandwich ELISA - Human CEBPB Antibody Pair - BSA and Azide free (ab244103)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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