Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-ABCA1 (phospho S2054) antibody [EPR2485]
See all ABCA1 primary antibodies
Description

Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)
Host species

Rabbit
Specificity

ab125064 only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
Database link: O95477
Positive control
- WB: Okadaic Acid and Calyculin A treated SH-SY5Y cell lysates. ICC/IF: Okadaic Acid and Calyculin A treated SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2485
Isotype

IgG
Research areas

Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/200000 dilution

Lane 1 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 (pS2054) peptide
Lane 2 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 non-phospho peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 254 kDa
Observed band size: 240 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% BSA/TBST.
Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/50000 dilution

Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 254 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

ab125064, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.
Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

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