Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ABCA1 (phospho S2054) antibody [EPR2485]
    See all ABCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2485] to ABCA1 (phospho S2054)

  • Host species

    Rabbit

  • Specificity

    ab125064 only detects ABCA1 phosphorylated at serine 2054. Cross reactivity with mouse was determined by sequence analysis only.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ABCA1 aa 2050-2150 (phospho S2054). The exact sequence is proprietary.
    Database link: O95477

  • Positive control

    • WB: Okadaic Acid and Calyculin A treated SH-SY5Y cell lysates. ICC/IF: Okadaic Acid and Calyculin A treated SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/200000 dilution

    Lane 1 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 (pS2054) peptide
    Lane 2 : SH-SY5Y whole cell lysate - treated with Okadaic Acid and Calyculin A with ABCA1 non-phospho peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 254 kDa
    Observed band size: 240 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    Western blot - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    All lanes : Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064) at 1/50000 dilution

    Lane 1 : SH-SY5Y cell lysate, untreated
    Lane 2 : SH-SY5Y cell lysate, Okadaic Acid and Calyculin A treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 254 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    ab125064, at a 1/500 dilution, staining ABCA1 (green) in Okadaic Acid and Calyculin A treated SH-SY5Y cells by immunofluorescence. DNA is marked in blue.
  • Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)
    Anti-ABCA1 (phospho S2054) antibody [EPR2485] (ab125064)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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