GATA-4 Transcription Factor Assay Kit (Colorimetric) (ab207206)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 1000 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 1 x 11ml 10X Wash Buffer 1 x 22ml 1 x 110ml 96-well GATA assay plate 1 unit 5 units Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 1 x 55µl Binding Buffer 1 x 10ml 1 x 50ml Developing Solution 1 x 11ml 1 x 55ml Dithiothreitol (DTT) (1 M) 1 x 100µl 1 x 500µl F9 nuclear extract (2.5 μg/μL) 1 x 40µl 1 x 200µl GATA-4 antibody 1 x 11µl 1 x 55µl Herring sperm DNA (1 μg/μL) 1 x 100µl 1 x 500µl Lysis Buffer 1 x 10ml 1 x 50ml Mutated oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl Plate sealer 1 unit 5 units Protease Inhibitor Cocktail 1 x 100µl 1 x 500µl Stop Solution 1 x 11ml 1 x 55ml Wild-type oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl -
Research areas
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Function
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735). -
Involvement in disease
Atrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Post-translational
modificationsMethylation at Lys-300 attenuates transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ASD2
- GATA 4
- GATA binding protein 4
see all -
Database links
- Entrez Gene: 2626 Human
- Omim: 600576 Human
- SwissProt: P43694 Human
- Unigene: 243987 Human
Images
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Different amounts of nuclear extracts from untreated (Gray) and Retinoic acid treated (Black) F9 cells are tested for GATA-4 activation by using the GATA-4 Transcription Factor Assay Kit.
Different amounts of untreated (grey) and retinoic acid treated (black) F9 cells were tested for GATA-4 activation. This data is provided for demonstration purposes only.