Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21107] to Axl
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Axl antibody [EPR21107]
See all Axl primary antibodies
Description

Rabbit monoclonal [EPR21107] to Axl
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: DU 145, NCI-H1299, 4T1, C2C12, HeLa and HUVEC whole cell lysates; Human skeletal muscle lysate; Rat and mouse muscle lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21107
Isotype

IgG
Research areas

Western blot - Anti-Axl antibody [EPR21107] (ab215205)

All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate at 40 µg
Lane 2 : AXL knockout HeLa cell lysate at 40 µg
Lane 3 : Wild-type HeLa cell lysate at 20 µg
Lane 4 : AXL knockout HeLa cell lysate at 20 µg

Performed under reducing conditions.

Predicted band size: 98 kDa
Observed band size: 135 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab215205 observed at 135 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab215205 was shown to react with Axl in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab215205 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Axl antibody [EPR21107] (ab215205)

All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : AXL knockout HeLa cell lysate
Lane 3 : NCI-H1299 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 98 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab215205 observed at 80,140 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab215205 Anti-Axl antibody [EPR21107] was shown to specifically react with Axl in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265392 (knockout cell lysate ab257152) was used. Wild-type and Axl knockout samples were subjected to SDS-PAGE. ab215205 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Axl antibody [EPR21107] (ab215205)

All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : AXL knockout HeLa cell lysate
Lane 3 : NCI-H1299 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 98 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab215205 observed at 80,140 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab215205 Anti-Axl antibody [EPR21107] was shown to specifically react with Axl in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261810 (knockout cell lysate ab257151) was used. Wild-type and Axl knockout samples were subjected to SDS-PAGE. ab215205 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Axl antibody [EPR21107] (ab215205)

All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

Lane 1 : DU 145 (human prostate carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 98 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?

Exposure time: 29 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Negative control: Jurkat
The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).

The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).
Western blot - Anti-Axl antibody [EPR21107] (ab215205)

All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate at 20 µg
Lane 2 : Rat muscle lysate at 10 µg
Lane 3 : Mouse muscle lysate at 10 µg
Lane 4 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate at 20 µg
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 7 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 98 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1: 6 seconds; Lane 2: 3 seconds; Lane 3: 46 seconds; Lanes 4-5: 3 minutes; Lanes 6-7: 10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).

The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).
Anti-Axl antibody [EPR21107] (ab215205)

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