Anti-Axl antibody (ab226927)
Key features and details
- Rabbit polyclonal to Axl
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Axl antibody
See all Axl primary antibodies -
Description
Rabbit polyclonal to Axl -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Recombinant fragment within Human Axl (internal sequence). The exact sequence is proprietary.
Database link: P30530 -
Positive control
- WB: HEK-293T cells expressing recombinant human Axl; H1299 and A549 whole cell lysates. IHC-P: Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human colon carcinoma tissue stained for Axl with ab226927 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Axl antibody (ab226927) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T transfected with human Axl, whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE
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All lanes : Anti-Axl antibody (ab226927) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 98 kDa5% SDS-PAGE