Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21107] to Axl - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Axl antibody [EPR21107] - BSA and Azide free
    See all Axl primary antibodies

  • Description

    Rabbit monoclonal [EPR21107] to Axl - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: DU-145, HeLa, and NCI-H1299 whole cell lysates.

  • General notes

    ab234258 is the carrier-free version of ab215205. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab234258 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : AXL knockout HeLa cell lysate
    Lane 3 : NCI-H1299 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab215205).

    Lanes 1-4: Merged signal (red and green). Green - ab215205 observed at 80,140 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab215205 Anti-Axl antibody [EPR21107] was shown to specifically react with Axl in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265392 (knockout cell lysate ab257152) was used. Wild-type and Axl knockout samples were subjected to SDS-PAGE. ab215205 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : AXL knockout HeLa cell lysate
    Lane 3 : NCI-H1299 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab215205).

    Lanes 1-4: Merged signal (red and green). Green - ab215205 observed at 80,140 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab215205 Anti-Axl antibody [EPR21107] was shown to specifically react with Axl in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261810 (knockout cell lysate ab257151) was used. Wild-type and Axl knockout samples were subjected to SDS-PAGE. ab215205 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate at 40 µg
    Lane 2 : AXL knockout HeLa cell lysate at 40 µg
    Lane 3 : Wild-type HeLa cell lysate at 20 µg
    Lane 4 : AXL knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 135 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab215205).

    Lanes 1 - 4: Merged signal (red and green). Green - ab215205 observed at 135 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    ab215205 was shown to react with Axl in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab215205 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    Western blot - Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : DU 145 (human prostate carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 98 kDa
    Observed band size: 140,80 kDa why is the actual band size different from the predicted?


    Exposure time: 29 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Jurkat
    The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).

    The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215205).

  • Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)
    Anti-Axl antibody [EPR21107] - BSA and Azide free (ab234258)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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