Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXA2 antibody [EPR4466] (ab108422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4466] to FOXA2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXA2 antibody [EPR4466]
    See all FOXA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4466] to FOXA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon cancer, fetal colon and mouse lung tissue lysates and HepG2 whole cell lysate (ab7900). IHC-P: Human hepatocellular carcinoma and mouse liver tissue; Human colon tissue; Mouse brain (substantia nigra) tissue. ICC/IF: HT-29 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    -ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    -ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal colon tissue lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422) This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human colon sections labelling FOXA2 with ab108422 at dilution of 1/500. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

    See Abreview

  • Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422) This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    ab108422 staining of FOXA2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Anti-FOXA2 antibody [EPR4466] (ab108422)
    Anti-FOXA2 antibody [EPR4466] (ab108422)

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