Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 02, 2021

Anti-FOXA2 antibody [EPR4466] (ab108422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4466] to FOXA2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-FOXA2 antibody [EPR4466]
See all FOXA2 primary antibodies
Description

Rabbit monoclonal [EPR4466] to FOXA2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon cancer, fetal colon and mouse lung tissue lysates and HepG2 whole cell lysate (ab7900). IHC-P: Human hepatocellular carcinoma and mouse liver tissue; Human colon tissue; Mouse brain (substantia nigra) tissue. ICC/IF: HT-29 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4466
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)

Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.
-ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
-ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)

All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal colon tissue lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)

Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422) This image is courtesy of Carl Hobbs, King's College London, United Kingdom

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human colon sections labelling FOXA2 with ab108422 at dilution of 1/500. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.
See Abreview
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)

Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422) This image is courtesy of Carl Hobbs, King's College London, United Kingdom

ab108422 staining of FOXA2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Anti-FOXA2 antibody [EPR4466] (ab108422)

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