Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16615-341] to IL-2 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free
    See all IL-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16615-341] to IL-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: EL4.IL-2 cells. Flow Cyt:EL4.IL-2 (mouse lymphoma T lymphocyte) treated with 80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin for 6 h, Mouse splenocytes treated with 80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin.

  • General notes

    ab278101 is the carrier-free version of ab243650. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278101 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16615-341

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
    Immunocytochemistry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)

    This data was developed using ab243650, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4.IL-2 cells labelling IL-2 with ab243650 at 1/50 (9.1 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in EL4.IL-2 cell line treated with Cell Stimulation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
    Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)

    This data was developed using ab243650, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with cell stimulation cocktail(80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours (Right)/ Untreated control (Left). cells labelling IL-2 with ab243650 at 1/500 dilution (1ug)/ Left and Right (Red) compared with a isotype control. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to FITC. Then fixed with 2% PFA for 10min followed by intracellularly stained with ab243650. Gated on lymphocytes population. Data was kindly provided by an anonymous collaborator.

  • Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
    Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)

    This data was developed using ab243650, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) treated with cell stimulation cocktail(80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours (Red)/ Untreated control (Green) cells labelling IL-2 with ab243650 at 1/50 dilution (1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
    Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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