Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
![Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)](https://www.abcam.com/ps/products/278/ab278101/Images/ab278101-1-anti-il2-antibody-epr16615341-immunocytochemistry-el4il2.jpg "Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16615-341] to IL-2 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse
Overview
-
Product name
Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free
See all IL-2 primary antibodies -
Description
Rabbit monoclonal [EPR16615-341] to IL-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC: EL4.IL-2 cells. Flow Cyt:EL4.IL-2 (mouse lymphoma T lymphocyte) treated with 80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin for 6 h, Mouse splenocytes treated with 80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin.
-
General notes
ab278101 is the carrier-free version of ab243650. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab278101 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16615-341 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)
This data was developed using ab243650, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4.IL-2 cells labelling IL-2 with ab243650 at 1/50 (9.1 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in EL4.IL-2 cell line treated with Cell Stimulation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
![Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)](https://www.abcam.com/ps/products/278/ab278101/Images/ab278101-3-anti-il2-antibody-epr16615341-flow-cytometry-mouse-splenocytes-treated-with-cell-stimulation-cocktail.jpg)
Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)This data was developed using ab243650, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with cell stimulation cocktail(80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours (Right)/ Untreated control (Left). cells labelling IL-2 with ab243650 at 1/500 dilution (1ug)/ Left and Right (Red) compared with a isotype control. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to FITC. Then fixed with 2% PFA for 10min followed by intracellularly stained with ab243650. Gated on lymphocytes population. Data was kindly provided by an anonymous collaborator.
-
![Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)](https://www.abcam.com/ps/products/278/ab278101/Images/ab278101-2-anti-il2-antibody-epr16615341-flow-cytometry-el4il2.jpg)
Flow Cytometry - Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)This data was developed using ab243650, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) treated with cell stimulation cocktail(80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours (Red)/ Untreated control (Green) cells labelling IL-2 with ab243650 at 1/50 dilution (1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)](https://www.abcam.com/ps/products/278/ab278101/Images/ab278101-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-2 antibody [EPR16615-341] - BSA and Azide free (ab278101)