Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16424] to Pigu - C-terminal
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Pigu antibody [EPR16424] - C-terminal
See all Pigu primary antibodies
Description

Rabbit monoclonal [EPR16424] to Pigu - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, 293, Human fetal brain, C6, RAW 264.7, PC12 and NIH 3T3 lysates; Human liver and mouse kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16424
Isotype

IgG
Research areas

Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/10000 dilution + HeLa cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution

Lane 1 : HT-1376 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)

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