Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)

Key features and details

  • Mouse monoclonal [27B2] to Desmoglein 1/DSG1 - BSA and Azide free
  • Suitable for: IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free
    See all Desmoglein 1/DSG1 primary antibodies

  • Description

    Mouse monoclonal [27B2] to Desmoglein 1/DSG1 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Human
    See all applications and species data

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IHC-Fr: Human normal skin.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product was previously labelled as Desmoglein 1.

    ab269569 is the carrier-free version of ab12077. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)
    Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)

    IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077. 

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab12077).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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