Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)
![Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)](https://www.abcam.com/ps/products/269/ab269569/Images/ab269569-368333-antidesmoglein1-dsg1-antibody-27b2-immunohustochemistry-frozen-skin-human.jpg "Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)")
Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1 - BSA and Azide free
- Suitable for: IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free
See all Desmoglein 1/DSG1 primary antibodies -
Description
Mouse monoclonal [27B2] to Desmoglein 1/DSG1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Human
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- IHC-Fr: Human normal skin.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product was previously labelled as Desmoglein 1.
ab269569 is the carrier-free version of ab12077. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
27B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)
IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab12077).
